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KMID : 0381120220440050603
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Genes and Genomics
2022 Volume.44 No. 5 p.603 ~ p.615
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Genome-wide DNA methylation profiling in differentiating Crohn’s disease from intestinal tuberculosis
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Wu Hao
Liu Hongchun
Liu Haining
Chen Yanjie
Liu Taotao
Shen Xizhong
Liu Lili
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Abstract
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Background: Differential diagnosis of Crohn’s disease (CD) and intestinal tuberculosis (ITB) is still difficult in clinical pratice. DNA methylation has been considered as a favorable area for biomarker exploration and identification.
Objective: The purpose of the current study was to evaluate DNA methylation changes between CD and ITB.
Methods: We performed a genome-wide association study to identify differentially methylated positions (DMPs), including 8 CD patients (before the initial of biologics or immunomodulators), 6 ITB patients, and 8 healthy controls (HCs), in whole blood DNA using the Infinium HumanMethylation850 BeadChip.
Results: Patients in the CD group and ITB group were all observed with hypo-methylated changes compared with HCs. However, the CD group overlaps with the ITB group in DNA methylation, suggesting a stable epigenetic profile between the two diseases. The pathway enrichment analysis showed the alternation in inflammation-related pathway, immune system, and signal transduction. Focused on the DMPs located in the promoter region, further analysis indicated hypermethylation of cg03122532 (5′UTR of KCNJ15) could be a potential CD-specific biomarker.
Conclusions: We identified specific differential methylation loci related to CD and ITB in blood DNA. DNA metylation as a important epigenetic modification could contribute to the pathogenesis study and biomarker exploration of the diseases.
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KEYWORD
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DNA methylation, Differentially methylated CpG sites, Crohn’s disease, Intestinal tuberculosis
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